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Effects of JAK1 and <t>JAK2</t> selective inhibitors on mechanical alloknesis in aged mice. (a) Schematic procedure for the mechanical alloknesis assay following 2 oral JAK inhibitors: JAK1 selective inhibitor (abrocitinib) and JAK2 selective inhibitor (AZ960) in aged mice. (b) Effects of abrocitinib ( n = 7) and AZ960 ( n = 7) on mechanical alloknesis in aged mice. DMSO was used as a vehicle control ( n = 13). Each dot represents an individual value, and the horizontal line in the middle of scattergrams indicates the mean value. Error bars indicate SEM. ** p < 0.01, ns: not significant (Dunnett's multiple comparison test).
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Effects of JAK1 and <t>JAK2</t> selective inhibitors on mechanical alloknesis in aged mice. (a) Schematic procedure for the mechanical alloknesis assay following 2 oral JAK inhibitors: JAK1 selective inhibitor (abrocitinib) and JAK2 selective inhibitor (AZ960) in aged mice. (b) Effects of abrocitinib ( n = 7) and AZ960 ( n = 7) on mechanical alloknesis in aged mice. DMSO was used as a vehicle control ( n = 13). Each dot represents an individual value, and the horizontal line in the middle of scattergrams indicates the mean value. Error bars indicate SEM. ** p < 0.01, ns: not significant (Dunnett's multiple comparison test).
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Effects of JAK1 and <t>JAK2</t> selective inhibitors on mechanical alloknesis in aged mice. (a) Schematic procedure for the mechanical alloknesis assay following 2 oral JAK inhibitors: JAK1 selective inhibitor (abrocitinib) and JAK2 selective inhibitor (AZ960) in aged mice. (b) Effects of abrocitinib ( n = 7) and AZ960 ( n = 7) on mechanical alloknesis in aged mice. DMSO was used as a vehicle control ( n = 13). Each dot represents an individual value, and the horizontal line in the middle of scattergrams indicates the mean value. Error bars indicate SEM. ** p < 0.01, ns: not significant (Dunnett's multiple comparison test).
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Effects of JAK1 and <t>JAK2</t> selective inhibitors on mechanical alloknesis in aged mice. (a) Schematic procedure for the mechanical alloknesis assay following 2 oral JAK inhibitors: JAK1 selective inhibitor (abrocitinib) and JAK2 selective inhibitor (AZ960) in aged mice. (b) Effects of abrocitinib ( n = 7) and AZ960 ( n = 7) on mechanical alloknesis in aged mice. DMSO was used as a vehicle control ( n = 13). Each dot represents an individual value, and the horizontal line in the middle of scattergrams indicates the mean value. Error bars indicate SEM. ** p < 0.01, ns: not significant (Dunnett's multiple comparison test).
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Effects of JAK1 and <t>JAK2</t> selective inhibitors on mechanical alloknesis in aged mice. (a) Schematic procedure for the mechanical alloknesis assay following 2 oral JAK inhibitors: JAK1 selective inhibitor (abrocitinib) and JAK2 selective inhibitor (AZ960) in aged mice. (b) Effects of abrocitinib ( n = 7) and AZ960 ( n = 7) on mechanical alloknesis in aged mice. DMSO was used as a vehicle control ( n = 13). Each dot represents an individual value, and the horizontal line in the middle of scattergrams indicates the mean value. Error bars indicate SEM. ** p < 0.01, ns: not significant (Dunnett's multiple comparison test).
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Effects of JAK1 and <t>JAK2</t> selective inhibitors on mechanical alloknesis in aged mice. (a) Schematic procedure for the mechanical alloknesis assay following 2 oral JAK inhibitors: JAK1 selective inhibitor (abrocitinib) and JAK2 selective inhibitor (AZ960) in aged mice. (b) Effects of abrocitinib ( n = 7) and AZ960 ( n = 7) on mechanical alloknesis in aged mice. DMSO was used as a vehicle control ( n = 13). Each dot represents an individual value, and the horizontal line in the middle of scattergrams indicates the mean value. Error bars indicate SEM. ** p < 0.01, ns: not significant (Dunnett's multiple comparison test).
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Effects of JAK1 and <t>JAK2</t> selective inhibitors on mechanical alloknesis in aged mice. (a) Schematic procedure for the mechanical alloknesis assay following 2 oral JAK inhibitors: JAK1 selective inhibitor (abrocitinib) and JAK2 selective inhibitor (AZ960) in aged mice. (b) Effects of abrocitinib ( n = 7) and AZ960 ( n = 7) on mechanical alloknesis in aged mice. DMSO was used as a vehicle control ( n = 13). Each dot represents an individual value, and the horizontal line in the middle of scattergrams indicates the mean value. Error bars indicate SEM. ** p < 0.01, ns: not significant (Dunnett's multiple comparison test).
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Effects of JAK1 and JAK2 selective inhibitors on mechanical alloknesis in aged mice. (a) Schematic procedure for the mechanical alloknesis assay following 2 oral JAK inhibitors: JAK1 selective inhibitor (abrocitinib) and JAK2 selective inhibitor (AZ960) in aged mice. (b) Effects of abrocitinib ( n = 7) and AZ960 ( n = 7) on mechanical alloknesis in aged mice. DMSO was used as a vehicle control ( n = 13). Each dot represents an individual value, and the horizontal line in the middle of scattergrams indicates the mean value. Error bars indicate SEM. ** p < 0.01, ns: not significant (Dunnett's multiple comparison test).

Journal: Acta Dermato-Venereologica

Article Title: Model of Chronic Itch in Aged Mice: Beneficial Effects of Drugs Affecting Descending Modulatory Systems

doi: 10.2340/actadv.v104.39950

Figure Lengend Snippet: Effects of JAK1 and JAK2 selective inhibitors on mechanical alloknesis in aged mice. (a) Schematic procedure for the mechanical alloknesis assay following 2 oral JAK inhibitors: JAK1 selective inhibitor (abrocitinib) and JAK2 selective inhibitor (AZ960) in aged mice. (b) Effects of abrocitinib ( n = 7) and AZ960 ( n = 7) on mechanical alloknesis in aged mice. DMSO was used as a vehicle control ( n = 13). Each dot represents an individual value, and the horizontal line in the middle of scattergrams indicates the mean value. Error bars indicate SEM. ** p < 0.01, ns: not significant (Dunnett's multiple comparison test).

Article Snippet: Bepotastine besilate, a selective histamine H 1 -receptor antagonist (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan); neurotropin, an extract from inflamed rabbit skin following vaccination with vaccinia virus (Nippon Zoki Co. Ltd, Oosaka, Japan); baricitinib, the JAK1/2 inhibitor (MedChem Express, Monmouth Junction, NJ, USA); pregabalin methyl ester, an antagonist of the voltage-dependent calcium channel α2δ subunit (Cayman Chemical, Ann Arbor, MI, USA and FUJIFILM Wako Pure Chemical Corporation), abrocitinib, the JAK1 selective inhibitor (Selleckchem, Houston, TX, USA) and AZ960, the JAK2 selective inhibitor (Selleckchem) were used as antipruritics.

Techniques: Control, Comparison